HL5-C Medium without Glucose supplemented with vitamins and micro elements Size:6 x 1kg Yeast extract 10 Peptone 20

Yeast extract 10 Peptone 20 Maltose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

Cold Spring Harbour laboratory

Threonine are slightly increased as well in SIH

soluble in ethanol

Besides these components

HL5-C Medium without Glucose supplemented with vitamins and micro elements Size:6 x 1kg Yeast extract 10 Peptone 20HL5 C supplemented with vitamins and micro elements as present in FM and SIH media. The addition of extra vitamins and micro elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis. Component g l Peptone 5 Yeast Extract 5 Tryptone 5 KH2PO4 1. 2 Na2HPO4 0. 35 FM Vitamins and Micro elements 0. 01 Suspend 16. 55 gram powdered medium in 1 litre distilled water.